Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots

نویسندگان

  • María Alicia MARTOS
  • Emilce Roxana ZUBRESKI
  • Mariana COMBINA
  • Oscar Alfredo GARRO
  • Roque Alberto HOURS
چکیده

Received 11/9/2012 Accepted 7/3/2013 (005877) 1 Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Felix de Azara, 1552, CP 3300, Posadas, Misiones, Argentina, e-mail: [email protected] 2 Instituto Nacional de Tecnología Agropecuaria – INTA, EEA Mendoza, San Martín, 3853, CP 5507, Luján de Cuyo, Mendoza, Argentina 3 Universidad Nacional del Chaco Austral, Comandante Fernández, 755, CP 3700, Presidencia Roque Sáenz Peña, Chaco, Argentina 4 Centro de Investigación y Desarrollo en Fermentaciones Industriales – CINDEFI, CONICET La Plata, Facultad de Ciencias Exactas, Universidad Nacional de la Plata – UNLP, Calle, 47 y 115, CP 1900, La Plata, Argentina *Corresponding author Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots María Alicia MARTOS1*, Emilce Roxana ZUBRESKI1, Mariana COMBINA2, Oscar Alfredo GARRO3, Roque Alberto HOURS4

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Production of Pectinolytic Enzymes by the Yeast Wickerhanomyces anomalus Isolated from Citrus Fruits Peels

Wickerhamomyces anomalus is pectinolytic yeast isolated from citrus fruits peels in the province of Misiones, Argentine. In the present work, enzymes produced by this yeast strain were characterized, and polygalacturonase physicochemical properties were determined in order to evaluate the application of the supernatant in the maceration of potato tissues. W. anomalus was able to produce PG in l...

متن کامل

Isolation and Characterization of Polygalacturonase Produced by Tetracoccosporium sp.

Thirty-five fungal strains which isolated from vegetable wastes, were screened for the use of polygalacturonic acid as the sole carbon source. Twenty-five isolates were positive for polygalacturonase activity in cup-plate assay, as evidenced by clear hydrolysation zones. The most productive strain was determined by measuring clear zones formed around colonies stained with ruthenium red.</em...

متن کامل

Isolation and Identification of Yeast Strains Capable of Producing Single Cell Protein from Whey in Co-Cultures with Saccharomyces cerevisiae

In this study, twenty-five whey samples collected from dairy industries in the city of Isfahan. The sampleswere cultured on malt extract broth (MEB) and yeast extract glucose chloramphenicol agar (YGCA) media.Eleven yeast strains (designated M1 to M11) were isolated from the culture. The strains were identified bytheir morphological and physiological properties. Betagalactosid...

متن کامل

Evidence that polygalacturonase is a virulence determinant in Erwinia carotovora.

Polygalacturonase (PG) was purified from Erwinia carotovora EC. A hybrid cosmid, pSH711, that encodes PG activity but not pectate lyase activity was identified from an E. carotovora genomic library by an immunological screening method. A cell extract of Escherichia coli cells containing pSH711 was able to produce plant tissue maceration when spotted on carrot, potato, or turnip slices. In addit...

متن کامل

Isolation and Partial Characterization of a Bacterial Thermostable Polymethyl Galacturonase from a Newly Isolated Bacillus sp. strain BR1390

Background: Pectinases are pectin degrading class of enzymes including polygalacturonase (PG), polymethyl galacturonase (PMG), pectate lyase (PEL), and pectin esterase (PE) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices. Objectives: A highly methylated pectin degrading bacterium from soil covered with fruit wa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2013